【Product Name】

Quantitative Detection Kit for Hepatitis B virus DNA (PCR-Fluorescence Probing)

【Intended Use】

This kit is suitable for the quantitative detection of hepatitis B virus nucleic acid (HBV DNA) in human serum samples in vitro, and  the test results are used for the auxiliary diagnosis of hepatitis B. The test results are not the only indicators for the evaluation of the  patient's condition, and the condition must be comprehensively analyzed in combination with the patient's clinical manifestations  and other laboratory tests. 

Hepatitis B is a worldwide epidemic and is a group of infectious diseases characterized by liver damage caused by Hepatitis B Virus (HBV).It has the characteristics of strong infectivity, complex transmission route, wide prevalence and high incidence. The  main clinical manifestations are fatigue, loss of appetite, nausea, vomiting, hepatomegaly and abnormal liver function. After a  person is infected with HBV, those who have not cleared the virus for 6 months are called chronic HBV infection. Without  appropriate intervention, some infected persons may develop liver failure, cirrhosis and primary hepatocellular carcinoma.

【Principle】

This kit utilizes real-time fluorescent PCR detection technology, and uses specific primers and probes for HBV to achieve  quantitative detection of HBV DNA in serum samples. The kit is provided with an internal control（IC）, which can monitor whether  there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false  negative PCR.

【Sample Requirements】

Applicable sample type: serum specimens.

【Material Required but Not Provided】

Applicable Instrument: ABI7500, ABI 7500 Fast and other Real-time fluorescence PCR instrument with FAM, VIC channels. 

Vortex shaker, palm centrifuge, pipette are needed.

【Storage Conditions And Expiration Date】  

Store below 30°C. It is valid for 12 months.