Intended Use
The Deoxynivalenol ELISA Kit is a competitive ELISA for the qualitative or quantitative analysis of Deoxynivalenol in wheat, wheat flour, barley, corn, etc.
Use Principles
The Deoxynivalenol kit is an indirect competitive enzyme-labeled immunoassay. The Deoxynivalenol antigen is precoated on the wells. The precoated antigen compete the Deoxynivalenol antibody (antibody solution) with Deoxynivalenol in the sample, anti-Deoxynivalenol antibody binds to the Deoxynivalenol-HRP enzyme conjugate. Then pipe the substrate solution to the wells to convert the color.
The color of unknown samples is compared to the color of the standards and the Deoxynivalenol concentration of the samples is derived.
Cross Reactivity
DON..............................................................................100%
3-AcDON.....................................................................<70%
15-AcDON.....................................................................<1%
Oxynivalenol ...............................................................<1%

1. Take out the reagents from the refrigerator and allow them to reach room temperature (20-25℃) prior to running the test.
2. Place the appropriate number of test wells into a microwell holder. Be sure to re-seal unused wells in the zip-lock bag with desiccant stored at the temperature 2-8℃ (do not freeze).
3. Number:Number the microwells for the standard and samples separately. Running standard and samples in duplicate.
4. Using a pipette with disposable tips, add 50μL of standard or samples to the appropriate test wells. Be sure to use a clean pipet tip for each. Then dispense 50μL of Enzyme Conjugate into each test well. At last, dispense 50μL of antibody Solution into each test well.
5. Shake the plate gently to mix contents, cover the plate rack and incubate the test wells at 25℃ keeping away from light for 30 minutes.
6. Open the cover on the plate and dump the contents of the wells into an appropriate waste container. Fill the wells to overflowing with wash solution and dump (250μL each well). Repeat for a total of four to five washes (wait 10s between each wash).
7. Following the last wash, tap the inverted wells onto absorbent paper to remove the last of the wash solution.
8. Dispense 50μL Substrate A and 50μL Substrate B into each well in order.