ABScript II RT Master Mix for qPCR

ABScript II Reverse Transcriptase is an M-Mulv Reverse Transcriptase modified by genetic engineering, which reduces the activity of RNase H, improves the thermal stability and cDNA synthesis efficiency, and ensures the authenticity and repeatability of qPCR results. ABScript II RT Master Mix for qPCR is a reverse transcription detection reagent suitable for two-step RT-qPCR. 5X ABScript II RT Mix contains all the reagents needed for reverse transcription reaction (ABScript II Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H2O. The operation is simple. The volume of RNA templates can be added up to 80% of the total volume, which is very suitable for the reverse transcription reaction of low-concentration RNA templates. 5 x ABScript II RT Mix will not freeze in - 20 ° C, easy access.
This product is specially optimized for qPCR. Oligo (dT)23VN has a stronger ability to anchor mRNA containing Poly(A) than Oligo (dT)18, making reverse transcription more efficient. Proportional optimized Random Primers/Oligo (dT)23VN Primer Mix enables cDNA synthesis to start from each region of RNA transcript and has the same reverse transcription efficiency, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR, and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.