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Business Type: Manufacturer
Main Products:antibodymolecular enzymesELISA KITProteinCRO servicesIVD materials
ID:21121568848
Category: Medical & Health & Beauty Medicine
MOQ:80000.00 Other
Cloning Ligation Adaptor LigationT4 DNA LigaseEnzymes
T/T
| Components | Cat. No. |
|---|---|
| T4 DNA Ligase (High Conc.) | RM21500 |
| 10X T4 DNA ligase Reaction Buffer | RM20108 |
| Featured | Specification |
|---|---|
| Product Source | An E.coli strain that carries the T4 DNA ligase gene. |
| Applications | Cloning Ligation, Adaptor Ligation |
| Unit Definition | One unit is defined as the amount of enzyme required to ligate 50% ligase of HindIII digestion fragments of λ DNA (5′ DNA termini concentration of 0.12 μM, 300- μg/ml) in a total reaction volume of 20 μl over 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. |
| Storage Conditions | 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 @ 25°C |
| Storage Temperature | -20°C |
| Reaction Conditions | 1X T4 DNA Ligase Reaction Buffer. |
| Reaction Buffer or Mix | 1X T4 DNA Ligase Reaction Buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH7.5 @ 25°C |
| Heat Inactivation | 65°C for 10 min. |
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